温馨提示
详情描述
immunohistochemical是什么意思，immunohistochemical翻译
Immunohistochemical techniques are widely used in biomedical research and diagnostic pathology to study the distribution and localization of specific proteins within cells and tissues. These techniques involve the use of antibodies that bind to target proteins with high specificity, allowing for the visualization and characterization of these proteins in various biological samples. In this article, we will explore the principles and applications of immunohistochemistry, highlighting its importance in the field of molecular biology and healthcare.
The basic principle of immunohistochemistry involves the detection of specific proteins within cells or tissues using labeled antibodies. The process typically starts with the fixation and processing of the biological sample, which allows for the preservation and visualization of the target proteins. Next, the sample is incubated with a primary antibody, which specifically recognizes and binds to the target protein. The primary antibody is then detected using a secondary antibody that is labeled with a reporter molecule, such as an enzyme, fluorophore, or radioisotope. The labeled secondary antibody binds to the primary antibody, allowing for the visualization of the target protein under a microscope or other detection devices.
There are several types of immunohistochemical techniques, each with its own advantages and applications. One common method is enzyme
